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TAL Effector Nucleotide Targeter 2.0
Tools
TALEN Targeter
Old TALEN Targeter
TAL Effector Targeter
Target Finder
Paired Target Finder
About TALE-NT
Resources
Software
FAQs
Tutorials
Submit TALEN Targeter
Sequence
Enter up to 2 megabases total in any number of sequences. Sequences should be in FASTA format and include a header line that starts with a '>'. For larger data sets, attach a file instead of pasting text in.
Sequence File
Files must be less than
2 MB
.
Allowed file types:
fasta
.
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Use a Preset Architecture
Preset Architecture
- None -
Cermak et al., 2011 (spacer 15-24, RVDs = 15-20)
Miller et al., 2011 (spacer 15-20, RVDs = 15-20)
Mussolino et al., 2011 (spacer 12-15, RVDs = 15-20)
Li et al., 2011 (spacer 16-31, RVDs = 15-20)
View full citations
Provide Custom Spacer/RVD Lengths
Spacer
Minimum Spacer Length
Maximum Spacer Length
Repeat Array
Minimum Repeat Array Length
Maximum Repeat Array Length
G Substitute
*
NN
NH
New data show that NH binds G more specifically than NN (which the tool used previously). See
Cong et al. (2012)
and
Streubel et al. (2012)
.
Filter Options
*
Show TALEN pairs (hide redundant TALENs)
Show TALEN pairs targeting a specific site
Show all TALEN pairs (include redundant TALENs)
Reyon, et al. (2012)
recently provided evidence that the previously available design guidelines have little effect on TALEN efficiency. Therefore, they have been removed from this version. They are still available with the
old version
.
Cut Site Position
Streubel et al. guidelines
*
Off
On
Require at least 1/4 of bases in each TALE to be C+G and disallow runs of 6 or more A+T in each TALE, as suggested by
Streubel et al. (2012)
.
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Count Predicted Targets in a Genome / Promoterome
Pre-loaded Sequence
- None -
Arabidopsis thaliana (genome)
Brachypodium distachyon (genome)
Caenorhabditis elegans (genome)
Danio rerio (genome)
Drosophila melanogaster (genome)
Gasterosteus aculeatus (genome)
Homo sapiens (genome)
Mus musculus (genome)
Oryza sativa (genome)
Arabidopsis thaliana (promoterome)
Brachypodium distachyon (promoterome)
Caenorhabditis elegans (promoterome)
Danio rerio (promoterome)
Drosophila melanogaster (promoterome)
Gasterosteus aculeatus (promoterome)
Homo sapiens (promoterome)
Mus musculus (promoterome)
Oryza sativa (promoterome, RapDB)
Oryza sativa (promoterome, MSU6)
Oryza sativa (promoterome, MSU7)
Oryza sativa (promoterome, RapDB Exclusive Sequences)
Rattus norvegicus (promoterome)
Rattus norvegicus (genome)
Solanum lycopersicum (genome)
Genome / promoterome to count predicted target sites for.
Count Predicted Targets in an NCBI Sequence
NCBI ID
NCBI ID to count predicted target sites for. See
this page
for help finding a usable ID.
Scoring Matrix
*
Doyle et al.
Miller et al.
Yang et al.
Upstream Base
*
T only (Recommended)
C Only
T or C
Select the base that should precede target sites on the 5' end.
Email
You can, but are not required to, enter an email address here to notify once your results are ready.
Expires
*
One hour
One day
One week
How long your results will be available for downloading from the server. After this time, your results will be permanently deleted.
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